Gene and protein sequence of an influenza neuraminidase with hemagglutinin activity
Identifieur interne : 002442 ( Main/Exploration ); précédent : 002441; suivant : 002443Gene and protein sequence of an influenza neuraminidase with hemagglutinin activity
Auteurs : G. M. Air [États-Unis] ; L. R. Ritchie [États-Unis] ; W. G. Laver [Australie] ; P. M. Colman [Australie]Source :
- Virology [ 0042-6822 ] ; 1985.
English descriptors
- Teeft :
- Amino, Amino acid, Amino acid sequence, Amino acids, Antigenic sites, Buffer gradient, Cdna, Cdna copy, Coding information, Dideoxy method, Ecori sites, Hemagglutinating neuraminidase, Hemagglutinin, Influenza, Influenza virus, Influenza virus neuraminidase, Laver, Neuraminidase, Neuraminidase gene, Nucleic acids, Nucleotide, Nucleotide sequence, Nucleotide sequence coding, Other influenza, Peptide, Plasmid puc9, Primer, Protein sequence, Sequence analysis, Sequencing, Sialic acid binding site, Subtype sequences, Synthetic oligonucleotides, Tryptic peptides, Universal primer, Virology, Virus neuraminidase.
Abstract
Abstract: An influenza virus neuraminidase (NA) of the N9 subtype also has hemagglutinin (HA) activity (W. G. Laver, P. M. Colman, R. G. Webster, V. S. Hinshaw, and G. M. Air (1984), Virology, 137, 314–323). To determine sequence relationships between this NA and other known NA and HA subtype sequences, and as a necessary step toward a complete structure determination, we have cloned a full-length copy of the coding sequence of the N9 NA of influenza virus A/tern/Australia/G70C/75 into the plasmid pUC9 using SalI linkers. The gene was sequenced by directed subcloning into the single-stranded phage vectors M13mp19 and M13mp18 and use of the dideoxy procedure. Most of the NA sequence was also obtained by direct protein sequencing of tryptic peptides. The N9 NA has 43 and 44% homology when compared to N1 or N2 sequences, respectively. There is no significant homology to any known HA sequence, or to the HN protein of the paramyxovirus SV5. Like the other NA molecules, the N9 NA is anchored in the membrane by an N-terminal hydrophobic region, from which biologically active heads can be released by pronase.
Url:
DOI: 10.1016/0042-6822(85)90206-5
Affiliations:
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Colman</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:90954EB5614BF3EB8A03386AA4D4528D2A399DB3</idno><date when="1985" year="1985">1985</date><idno type="doi">10.1016/0042-6822(85)90206-5</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-GKFBQNWC-W/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000393</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000393</idno><idno type="wicri:Area/Istex/Curation">000393</idno><idno type="wicri:Area/Istex/Checkpoint">001124</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001124</idno><idno type="wicri:doubleKey">0042-6822:1985:Air G:gene:and:protein</idno><idno type="wicri:Area/Main/Merge">002563</idno><idno type="wicri:Area/Main/Curation">002442</idno><idno type="wicri:Area/Main/Exploration">002442</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Gene and protein sequence of an influenza neuraminidase with hemagglutinin activity</title><author><name sortKey="Air, G M" sort="Air, G M" uniqKey="Air G" first="G. M." last="Air">G. M. Air</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294</wicri:regionArea><wicri:noRegion>Alabama 35294</wicri:noRegion></affiliation></author><author><name sortKey="Ritchie, L R" sort="Ritchie, L R" uniqKey="Ritchie L" first="L. R." last="Ritchie">L. R. Ritchie</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294</wicri:regionArea><wicri:noRegion>Alabama 35294</wicri:noRegion></affiliation></author><author><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>John Curtin School of Medical Research, P.O. Box 334, Canberra City, ACT 2601</wicri:regionArea><wicri:noRegion>ACT 2601</wicri:noRegion></affiliation></author><author><name sortKey="Colman, P M" sort="Colman, P M" uniqKey="Colman P" first="P. M." last="Colman">P. M. Colman</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Commonwealth Scientific and Industrial Research Organization, Division of Protein Chemistry, 3.43 Royal Parade, Parkville, Victoria 3052</wicri:regionArea><wicri:noRegion>Victoria 3052</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">145</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="117">117</biblScope><biblScope unit="page" to="122">122</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid</term><term>Amino acid sequence</term><term>Amino acids</term><term>Antigenic sites</term><term>Buffer gradient</term><term>Cdna</term><term>Cdna copy</term><term>Coding information</term><term>Dideoxy method</term><term>Ecori sites</term><term>Hemagglutinating neuraminidase</term><term>Hemagglutinin</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Laver</term><term>Neuraminidase</term><term>Neuraminidase gene</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Nucleotide sequence coding</term><term>Other influenza</term><term>Peptide</term><term>Plasmid puc9</term><term>Primer</term><term>Protein sequence</term><term>Sequence analysis</term><term>Sequencing</term><term>Sialic acid binding site</term><term>Subtype sequences</term><term>Synthetic oligonucleotides</term><term>Tryptic peptides</term><term>Universal primer</term><term>Virology</term><term>Virus neuraminidase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An influenza virus neuraminidase (NA) of the N9 subtype also has hemagglutinin (HA) activity (W. G. Laver, P. M. Colman, R. G. Webster, V. S. Hinshaw, and G. M. Air (1984), Virology, 137, 314–323). To determine sequence relationships between this NA and other known NA and HA subtype sequences, and as a necessary step toward a complete structure determination, we have cloned a full-length copy of the coding sequence of the N9 NA of influenza virus A/tern/Australia/G70C/75 into the plasmid pUC9 using SalI linkers. The gene was sequenced by directed subcloning into the single-stranded phage vectors M13mp19 and M13mp18 and use of the dideoxy procedure. Most of the NA sequence was also obtained by direct protein sequencing of tryptic peptides. The N9 NA has 43 and 44% homology when compared to N1 or N2 sequences, respectively. There is no significant homology to any known HA sequence, or to the HN protein of the paramyxovirus SV5. Like the other NA molecules, the N9 NA is anchored in the membrane by an N-terminal hydrophobic region, from which biologically active heads can be released by pronase.</div></front></TEI><affiliations><list><country><li>Australie</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Air, G M" sort="Air, G M" uniqKey="Air G" first="G. M." last="Air">G. M. Air</name></noRegion><name sortKey="Ritchie, L R" sort="Ritchie, L R" uniqKey="Ritchie L" first="L. R." last="Ritchie">L. R. Ritchie</name></country><country name="Australie"><noRegion><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name></noRegion><name sortKey="Colman, P M" sort="Colman, P M" uniqKey="Colman P" first="P. M." last="Colman">P. M. Colman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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